Introduction
Immunoanalyzer is a common biochemical experimental equipment used to analyze trace substances in organisms, such as proteins, hormones, cytokines, etc. It is a machine based on the principle of immune response, which can quantitatively or semi-quantitatively determine specific biomolecules in a sample.
Detection Principles Of Immunoassay Analyzer
The immune analyzer can use the specific binding reaction between the antigen and the antibody for analysis. The operator adds the sample to the known antigen or antibody kit, and then adds a specific enzyme-labeled antibody. The enzyme-labeled antibody and the corresponding Antibodies combine to form a complex, and under specific conditions, the enzyme-labeled antibody is transformed into signals such as fluorescence and color, so as to quantitatively or semi-quantitatively determine the content of the substance to be tested. This instrument, which can convert the structure and characteristics of substances into visible and measurable spectral signals, has the characteristics of high detection sensitivity, fast and accurate, and convenient operation, and is not affected by the nature of the detected substance itself. One of the most important techniques in the detection principle.
Detection Methods Of Immunoassay Analyzer
Fluorescence immunoassay is a method of analyzing and detecting substances by using specific fluorescent properties of substances. When a substance is exposed to a light source of a certain energy, the substance will emit fluorescence, which is called excited fluorescence, and the wavelength of fluorescence is generally higher than the wavelength of excitation light. Similarly, the fluorescent immunoassay analyzer also uses the principle of excited fluorescence to realize the detection of substances, which includes multiple parts such as excitation source, detection system, amplification system and display system. First, the fluorescence immunoassay analyzer has an excitation source to provide a light source of a specific wavelength, which excites the substance into a visible spectral signal, and then the detection system converts the spectral signal into an electrical signal for better detection. The amplification system can amplify the detected electrical signal to make the detection result more obvious. Finally, the display system converts the amplified signal into an image or data and displays it, so as to realize the measurement and quantitative analysis of substances.
The more clinically used types include chemiluminescence immunoassay analyzer, microparticle chemiluminescence immunoassay analyzer and electrochemiluminescence immunoassay analyzer.
1. Chemiluminescence immunoassay analyzer
Including the host part and the microcomputer processing system. Chemiluminescent immunoassay technology, also known as micro-multiplication technology, includes two methods:
①Competition method: mostly used for the determination of small molecule antigenic substances. An excess of antibody coated with magnetic particles is added to the cuvette for incubation with the antigen to be tested and the quantified labeled antigen at the same time, so that the labeled antigen and antibody (or the antigen to be tested and antibody) combine to form a complex.
②Sandwich method: It is mostly used for the determination of macromolecular antigenic substances. The labeled antibody and the tested antigen combine with the coating antibody at the same time to form a complex of coating antibody-measurement antigen luminescence antibody. The instrument uses certain chemical groups to mark on the antigen or antibody. The chemical group is oxidized to form an excited state, and a photon of a certain wavelength is released during the process of returning to the ground state. The photomultiplier tube converts the received light energy into electrical energy. , to reflect the light measurement in digital form, and then calculate the concentration of the measured object.
2. Microparticle Chemiluminescence Immunoassay Analyzer
Including sample processing system, experiment operation system, central supply and control system and microcomputer control system. It applies classical immunological principles, uses monoclonal antibody reagents, uses magnetic particles as solid phase carriers, alkaline phosphatase as the standard substance, and uses 3-(2′-helixadamantane)-4-methoxy- For 4-(3″-phosphoryloxy)benzene-1,2-dioxetane (AMPPD), small molecular substances are determined by competition method or antibody capture method, while macromolecular substances are determined by sandwich method.
3. Electrochemiluminescence immunoassay analyzer
It is mainly composed of sample tray, reagent kit, incubation reaction tray, electrochemical detection system and computer control system, and can be divided into three unit modules (control unit, core unit and analysis module).
The sample to be tested is mixed with antibody-coated paramagnetic particles and luminescent agent-labeled antibody in a cuvette for co-incubation, and the formed microbeads are coated with antibody-antigen-luminescent agent-labeled antibody complex. When the magnetic particles flow through the surface of the electrode, they are attracted by the magnet installed under the electrode, and the free luminescent agent-labeled antibody is washed away by the buffer. At the same time, a voltage is applied to the electrode to make the luminescent marker ruthenium terpyridine [Ru(bpy)3]2+ transfer electrons on the surface of the electrode to generate electrochemiluminescence, and the intensity of the light is proportional to the concentration of the antigen to be tested. The suppression immunoassay is used for the detection of small molecular weight protein antigens; the sandwich immunoassay is used for the detection of large molecular weight substances.
Why Choose Us?
CNMEDITECH is dedicated to the long-term research of the immune analyzer market. Our mission is “People oriented and win-win strategy,Matching the real needs of the region with a focus on human health,To be the world’s first-class medical field solution expert”. We have been manufacturing high-quality medical device products for more than ten years.
We have built our reputation on delivering quality healthcare solutions on time and on budget. All our products comply with international health and safety regulations and all products come with a warranty.
Are you still worrying about your customer’s product needs? Are you still angry that the product is expensive?Our immune analyzers have various models and support personalized product customization.
Our company has many styles to choose from. In addition, we have high-quality pre-sales consulting guidance and professional after-sales service, all to meet your needs.
Whether it is a cost-effective or high-end product, there will always be something suitable for you. If you have any needs for products, you can ask us, our factory will meet your needs as soon as possible, and we will make every effort to provide you with solutions.Feel free to send us your inquiries.