Introduction

Immunoassays are analytical techniques that utilize the specific binding affinity between antibodies and antigens to quantitatively measure target molecules, such as proteins, hormones, drugs, and pathogens.It is based on the characteristic reactions of antigens and antibodies.Due to its advantages of high sensitivity, strong specificity, wide application, simple equipment required, and wide linear range, it is increasingly favored by people.

What Has Changed In Immunoassay Analyzer?

Enzyme Immunoassay (Enzyme Immunoassay, EIA) is an important technology in labeled immunoassay. It uses enzyme-labeled antibody (antigen) as the main reagent to combine the specificity of antigen-antibody reaction and the efficiency of enzyme-catalyzed substrate reaction with the An immunoassay technique that combines specificity. As one of the three classic labeling technologies, enzyme immunology technology has been widely used in laboratory medicine and has been continuously updated. It has been continuously integrated with other advanced technologies such as fluorescence, luminescence technology, and instrument automation. It is becoming more and more perfect. Many automated instruments are actually The complex of EIA technology and other modern technologies, its analytical sensitivity has reached or even greatly exceeded the level of radioimmunoassay (RIA), because the reagents are stable and free of radioactive pollution, and the analytical forms are increasingly diversified, simple and flexible, and widely used in clinical applications. Highly regarded.In recent years,enzyme immunoassay has made progress mainly in the following aspects:

1.The application of the third-generation antibody genetically engineered antibody significantly improves the specificity of detection, basically eliminates the non-specific cross-reaction between antigen and antibody, and ensures the accuracy of analysis. The advancement of antibody preparation technology has enabled the production and preparation of reagents to be scaled up, and the detection range has been increasingly expanded.

2.Developed double-antigen sandwich method for antibody detection, coupled enzyme labeling method for antigen detection, bridging enzyme immunoassay, enzyme amplification immunoassay technology, etc., applied biotin-avidin system, substrate cycle amplification system, enzyme- Anti-enzyme amplification system and enzyme coupling amplification system, etc.

3.The labeling enzyme has expanded from peroxidase to more than 20 kinds of enzymes that have been used in EIA so far, but the most widely used are still horseradish peroxidase (HRP) and alkaline phosphatase (Alkalinephasphotase) , ALP).

4.Combination of enzyme immunoassay and other marker immunoassays.

5.Improve the technology of solid phase carrier, increase the binding capacity of the solid phase surface, and increase the range of conjugates.

Enzyme immunoassay is integrated with modern technology in methodology, the development of modern biological technology, the improvement of antigen antibody preparation and enzyme labeling technology, especially the standardization of commercial kits and the application of automated instruments, making it a suitable Immunolabeling technology of inspection departments at all levels. With the continuous development of laboratory medicine, enzyme immunoassay has been developed through the integration of modern technology.

In automated instruments, enzyme immunoassay technology and other analytical techniques are also used in combination, such as chemiluminescent enzyme immunoassay, which is an enzyme-labeled immunoassay that uses chemiluminescent agents as enzyme reaction substrates. After enzymatic and luminescent two-stage amplification, it has high sensitivity. The name is VITROS Eci automatic optional enhanced chemiluminescence immunoassay system, which combines enzyme immunology technology, biotin avidin technology and enhanced chemiluminescence technology. The antigen or antibody is labeled with horseradish peroxidase, the plastic small hole tube is used as the solid phase carrier, and luminol is used as the chemiluminescence agent. The key technology is the application of a luminescent enhancer, which increases the luminous intensity by a thousand times, prolongs and stabilizes the time; enzymatic fluorescence amplification immunoassay technology, the system uses the double antibody sandwich method to label the antigen or antibody with alkaline phosphatase, and uses a plastic straw (SPR ) as a solid phase carrier, with 4-methylumbelliferone phosphate (4-MIjP) as a luminescent agent; microparticle enzyme immunoassay technology, scientific methods, stable reagents and instruments, high sensitivity, wide quantitative linear range, etc., such as the United States Abbott’s IMX automatic immunofluorescence analyzer, the reaction finally forms the antibody-analyte-alkaline phosphatase-labeled secondary antibody sandwich complex coated on the particle, which is transferred to the glass fiber column and washed with buffer, and then Substrate is added for determination, and supporting reagents are available.

In modern society, the automation of enzyme immunoassay technology is also developing at a high speed. In the 1950s, automatic biochemical analyzers were applied in practice. Due to the particularity of enzyme immunoassay technology, no automatic analysis system came out until the 1980s. According to the different analysis processes, enzyme immunoassays can be divided into two categories: homogenous and heterogenous.

It is undeniable that enzyme immunoassay technology also has certain limitations. First of all, the purity and specificity of the antigen and antibody used for coating in the reagent, the stability, specificity, purity, affinity and preparation process of the enzyme marker are affected by many factors, so the detection kits should be strictly compared and selected. Secondly, enzyme immunoassay is mainly based on solid phase reaction. In the measurement, attention should be paid to overcome the surface effect caused by the inconsistency of the amount of antigen (antibody) coated on different parts of the solid phase, and to prevent the edge effect caused by the inconsistency of the reaction conditions between the edge hole and the center hole during incubation. , and the hook effect that may be caused by the mismatch between the ratio of antigen and antibody. It must be noted that the easy-to-operate “one-step method” is often more prone to hook effects than the “two-step method”. In addition, solid-phase materials have non-specific adsorption, and peroxidase can be released from specimen hemolysis or storage in the refrigerator. Too long storage in the refrigerator can lead to aggregation of serum IgG, which can easily cause high background and even seriously interfere with the measurement. These drawbacks are evident in semi-automated instruments and in small specimen volumes requiring refrigerator storage.

Why Choose Us?

CNMEDITECH is dedicated to the long-term research of the immune analyzer market. Our mission is “People oriented and win-win strategy,Matching the real needs of the region with a focus on human health,To be the world’s first-class medical field solution expert”. We have been manufacturing high-quality medical device products for more than ten years.

We have built our reputation on delivering quality healthcare solutions on time and on budget. All our products comply with international health and safety regulations and all products come with a warranty.

Are you still worrying about your customer’s product needs? Are you still angry that the product is expensive?Our immune analyzers have various models and support personalized product customization.

Our company has many styles to choose from. In addition, we have high-quality pre-sales consulting guidance and professional after-sales service, all to meet your needs.

Whether it is a cost-effective or high-end product, there will always be something suitable for you. If you have any needs for products, you can ask us, our factory will meet your needs as soon as possible, and we will make every effort to provide you with solutions.Feel free to send us your inquiries.