Introduction
Hemoglobin A1c (HbA1c) analyzers are essential tools in the management of diabetes, providing valuable information about a patient’s long-term glucose control.However, like any complex medical instrument, they can experience malfunctions that affect their accuracy and reliability.
To ensure accurate and reliable results, it’s essential to adhere to several precautions when using these analyzers.
What Are The Common Malfunctions Of Glycosylated Hemoglobin Analyzers?
1. Water comes out of the liquid supply port
At this time, you can check whether the booster is blocked; whether the number of descending steps of the syringe needle is correct; whether the sealing ring of the liquid supply port is damaged, and then you can use a syringe to remove the blockage of the waste liquid pipe to see whether the waste liquid pipe discharges water droplets or water columns. If If there are water droplets, remove the blockage or replace the booster; check the number of descending steps of the syringe. If there is any problem, adjust the number of descending steps or replace the sealing ring of the liquid supply port.
2. The instrument does not inject samples
Check whether the pump tube is adhered. You can remove the pump tube, rub it, and then reinstall it. If the pump tube has not been replaced for a long time or the pump tube is aging, you can replace the pump tube. If the pump tube barrel is worn or stuck, you can replace the pump tube on the shaft of the pump tube. Grease the replacement pump tube shaft.
3. It prompts that TIME exceeds the limit and the printed report shows an error
Check whether the pump tube has been replaced, whether the connecting pipe between the pump tube and the bubble detection tube is blocked, replace the pump tube sleeve, and check whether the pump tube barrel is stuck, and perform corresponding blockage removal operations. If it is found that the pretreatment column has not been replaced, replace it. If the chromatography column fails, it needs to be replaced in time. If the pre-warmed blood is left for too long, the sample also needs to be reprocessed.
4. The absorbance exceeds the standard
Check whether the sample dilution ratio is correct, increase or decrease the dilution ratio and re-measure; observe whether there are bubbles in the colorimetric cell.
5. It prompts that the IC card is not detected, please insert the IC card
Re-insert and unplug the IC card; remove the back cover, re-insert the data cable, and re-insert and unplug the software.
6. It prompts that there are bubbles in the flow path or the reagent is used up
If the reagent is used up, it needs to be replaced. If there is a problem with the pump tube, it needs to be replaced. The pipeline and tee should be checked for air leakage. Check whether the valve is not open or closed tightly.
7. Prompt high potential abnormality
Check the high potential, observe whether there are bubbles in the colorimetric cell, unplug the data cable to see if there is any response, or replace the optical system.
8. The measurement sample value is low and inconsistent with clinical practice.
Check the difference between the low value and the actual blood sugar value, perform quality control for comparison, and recalibrate the system.
9. The measured value is very high or very low
Check whether the graph on the report is correct and what the value of the slope intercept is. If the intercept is wrong, recalibrate.
What Precautions Should Be Taken When Using Glycosylated Hemoglobin Analyzers?
1.Collection of standard books: Collect venous blood according to conventional methods for clinical laboratory testing, or blood from terminal capillaries. Use blood collection tubes containing ethylenediaminetetraacetic acid (EDTA) anticoagulant or use blood collection tubes according to the manufacturer’s requirements. , please note that severely hemolyzed and severely turbid specimens will interfere with the reaction absorbance and affect the test results;
2.Specimen storage and transportation: Specimens should be transported to the testing room in a timely manner after collection. Whole blood specimens are stable for 1 week when stored at 4°C, but are not suitable for long-term storage at -20°C. Specimens that have been tested should be stored at 2-8°C for 1 week. Prepare for reexamination of abnormal conditions; improper handling of specimens, such as placing them at high temperatures for a long time, will introduce a large number of unknown interference factors, and the degree of interference depends on the method used;
3.Experimental operations must be standardized: strictly follow the experimental standard operating procedures, store reagents and consumables in accordance with regulations, use them within the validity period, and the instruments must be regularly maintained.
Conclusion
In conclusion, HbA1c analyzers play a vital role in diabetes management, but they are not immune to malfunctions. Understanding and addressing the common malfunctions outlined in this article will contribute to more accurate HbA1c test results.By adhering to these precautions, healthcare professionals can ensure the accurate and reliable operation of HbA1c analyzers, contributing to better diabetes management and improved patient care.
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